Evaluation of Antimicrobial Efficacy of Nano Chitosan and Chlorohexidine versus Sodium Hypochlorite as Final Rinse against Enterococcus Faecalis

Authors

Sarah Mohamed Nour ElDeen, Dental Practitioner in Governmental Medical Center in El Sherouk CityFollow
wael kamel, Professor and head of Endodontic Department Faculty of Dental Medicine, Future University, Professor of Endodontic Department Faculty of Dental Medicine for Girls Al-Azhar University, Cairo, Egypt.Follow
Mohammed Rokaya, Associate Professor and head of Endodontic Department Faculty of Dentistry, Horus University, Egypt, Associate Professor of Endodontic, Endodontic Department Faculty of Dental Medicine, Al-Azhar University- Assiut Branch, Egypt.Follow
Mohamed Aly Sherif, Professor of Microbiology and Immunology Faculty of Medicine, Al-Azhar University, Cairo, Egypt.

Corresponding Author

Mohamed Nour ElDeen, Sarah

Document Type

Original Study

Abstract

Purpose: This study aimed to evaluate the antimicrobial efficacy of nano chitosan and chlorohexidine versus sodium hypochlorite as final rinse against Enterococcus faecalis. Methods: Forty single rooted teeth were selected. All root canals were prepared manually to #50 file. Finally, the teeth were sterilized by gamma rays. Each root canal was inoculated with 0.5 ml of E. faecalis. Specimens were divided randomly into 4 main groups (10 specimens each) according to the irrigating solutions. Group 1: nano chitosan 0.2%, group 2: Chlorhexidine 2%, group 3: alternating solution of nano chitosan 0.2% & Chlorhexidine 2%, and group 4: Sodium hypochlorite 3%. The mean of bacterial count before and after final irrigation by the tested solutions were measured. Results: The highest mean difference value of bacterial count was found in 3% sodium hypochlorite, followed by alternating solution of 0.2% nano chitosan & 2% chlorhexidine, then 2% chlorhexidine, and 0.2% nano chitosan came lastly. However, there was no statistically significant difference among the tested groups. Conclusions: alternating solution of 0.2% nano chitosan & 2% chlorhexidine when used as antibacterial irrigant gives promising results and can be used as an alternative to NaOCl solution.

Keywords

nano chitosan; Chlorohexidine; Enterococcus faecalis; Final rinse

How to Cite This Article

Mohamed Nour ElDeen, Sarah; kamel, wael; Rokaya, Mohammed; and Aly Sherif, Mohamed (2023) "Evaluation of Antimicrobial Efficacy of Nano Chitosan and Chlorohexidine versus Sodium Hypochlorite as Final Rinse against Enterococcus Faecalis," Al-Azhar Journal of Dentistry: Vol. 10: Iss. 1, Article 4.
DOI: https://doi.org/10.58675/2974-4164.1460

Subject Area

Restorative Dentistry Issue (Removable Prosthodontics, Fixed Prosthodontics, Endodontics, Dental Biomaterials, Operative Dentistry)