Evaluation of the Effect of Different Concentrations of Rosehip on Gingival Fibroblastic Cell Culture

Authors

Mai H. Mohamed, Dentist at Egyptian Ministry of Health.Follow
Naglaa Shawki Elkilani, Professor and Head of Oral Medicine, Periodontology, Oral Diagnosis and Oral Radiology Department Faculty of Dental Medicine for Girls Al-Azhar University, Cairo, Egypt.
Entsar E. Badr, Professor of Applied organic Chemistry, chemistry Department, Faculty of Science for Girls Al-Azhar University, Cairo, Egypt
Omneya E . Ahmed, Lecturer of Oral Medicine, Periodontology, Oral Diagnosis and Radiology, Faculty of Dental Medicine for Girls Al-Azhar University, Cairo, Egypt.

Document Type

Original Study

Abstract

Purpose: The purpose of this study was to see how varied rosehip concentrations affected gingival fibroblastic cell culture. Materials and Methods: we prepared different concentrations of Rosehip oil (RHO) and RHO with Solvent (RHO/S). Gingival fibroblast cells were isolated from gingivitis patient. Gingival fibroblast cells were cultured, characterized, and analyzed in two subgroups. 1st subgroup: gingival fibroblast cells were treated with RHO RHO\S at different concentrations (5, 10) µg/mL, counted using a hemocytometer. Trypan blue and the cell proliferation assay3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) were done at 24, 48, 72hours. The half-maximal effective concentration (EC50) of RHO and RHO/S was calculated, and apoptosis was detected after 72 hours of treatment with RHO and RHO/S (EC50). 2nd subgroup: untreated cells. Results: A statically significant (P≤0.05) positive correlation was observed between different concentrations of RHO, RHO\S, cell count, cell proliferation and viability. Conclusion: RHO and RHO/S can be effectively used on gingival fibroblast cells; no in vitro cytotoxicity was detected for RHO and RHO/S.

Keywords

Rosehip oil, gingival fibroblast cells, apoptosis.

How to Cite This Article

Mohamed, Mai H.; Elkilani, Naglaa Shawki; Badr, Entsar E.; and Ahmed, Omneya E . (2023) "Evaluation of the Effect of Different Concentrations of Rosehip on Gingival Fibroblastic Cell Culture," Al-Azhar Journal of Dentistry: Vol. 10: Iss. 3, Article 3.
DOI: https://doi.org/10.58675/2974-4164.1549

Subject Area

Oral Medicine and Surgical Sciences Issue (Oral Medicine, Oral and Maxillofacial Surgery, Oral Pathology, Oral Biology)