Effect of Protein Repellent Addition to Self-Healing Experimental Resin Composite on Microcosm Biofilm and Cytotoxicity

Authors

Passant Abdel Hameed, Associate Lecturer of Dental Biomaterials, Al Zagazig University, EgyptFollow
Inas Motawea, Professor of Dental Biomaterials, Faculty of Dental Medicine for Girls, Al-Azhar University, Cairo, EgyptFollow
Hisham Essawy, Professor in department of Polymer and Pigment chemistry, National Research Center, Cairo, EgyptFollow
Mohamed Hosny, Professor of Microbiology, Regional Center for Mycology and Biotechnology, Al-Azhar University, Cairo, EgyptFollow

Corresponding Author

Abdel Hameed, Passant

Document Type

Original Study

Abstract

Purpose: To investigate the effect of addition of MPC protein repellent agent to selfhealingexperimental resin composite on the self-healing efficacy, microcosm biofilmand the cytoxicity. Material and Methods: Polyurea formaldehyde microcapsuleswere synthesized by Insitu Polymerization Technique. MPC and the microcapsules areadded to the prepared experimental resin composite with different MPC concentrations.Protein repellence essay was examined by the Micro bicinchoninic Acid method.Biofilm models formed of streptococcus mutans growth was prepared and visualized byconfocal laser electron microscope to investigate the live \ dead assay over the studiedspecimens. The cytotoxicity of the specimens was examined via SRB assay. Fracturetoughness was examined using universal testing machine till fracture. Self-healingefficacy is evaluated. Results: In protein deposition, it was found that the 0%MPChas the highest value while the least value was found in the 10% group. In live/dead bacterial assay, it was found that the 0%MPC has the highest living bacterialvalue and the lowest dead bacterial value while the 7.5% group has the lowest livingbacterial value and the highest dead bacterial value. In the mechanical evaluation,the virgin fracture toughness, the highest facture toughness was found in the controlspecimens while the least fracture toughness was found in 10% group, the selfhealingefficacy results showed that the highest self-healing value was found in the0%MPC while the least self-healing efficacy value was found in the control group.Conclusion: Innovative self-healing composite with protein repellence give rise fora great variety of dental biomaterials with more promising results and long clinicalserviceability

Keywords

Self-healing; Protein repellent; Biofilm models; confocal electron scanning; Cytotoxicity

How to Cite This Article

Abdel Hameed, Passant; Motawea, Inas; Essawy, Hisham; and Hosny, Mohamed (2023) "Effect of Protein Repellent Addition to Self-Healing Experimental Resin Composite on Microcosm Biofilm and Cytotoxicity," Al-Azhar Journal of Dentistry: Vol. 10: Iss. 2, Article 18.
DOI: https://doi.org/10.58675/2974-4164.1505

Subject Area

Restorative Dentistry Issue (Removable Prosthodontics, Fixed Prosthodontics, Endodontics, Dental Biomaterials, Operative Dentistry)