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Corresponding Author

Yehia, Aya

Document Type

Original Study

Abstract

Purpose: Current study proposed to estimate the anticarcinogenic influence of Selenium Nanoparticles (SeNps) on induced squamous cell carcinoma utilizing caspase3 as an apoptotic marker. Material and Methods: Thirty hamsters were used, divided into four groups. Group I: 2 hamsters were left untreated, Group II: 8 hamsters received SeNPs orally for 14 weeks at dosage of 0.5 mg/kg on alternative day along the experimental period. Group III: 10 hamsters received Dimethylbenz [a]anthracene (DMBA) (0.5%) painted by brush on the buccal pouch 3 times per week for 8 weeks, then received DMBA with 5-Fluorouracil (5-FU) for another 6 weeks twice a week at dosage of 12.5 mg/kg and continued on 5-FU treatment for another 2 weeks after stop of DMBA. Group IV: 10 hamsters received DMBA alone for 4 weeks. Then received DMBA along with SeNPs for 10 weeks at dosage of 0.5 mg /kg on alternative days to DMBA, continued on SeNPs treatment for another 2 weeks after stop of DMBA. Results: Comparing all groups according to caspase 3 expression revealed that the mean area percentage values have been arranged with the following descending pattern group III, group IV, group I then group II. ANOVA test showed a statistically significant variance between groups, with no statistically significant difference between group I and group II together showed the statistically significant lowest mean area percent values or group III and group IV together showed statistically significant highest mean area percent values. Conclusion: Chitosan coated SeNPs is a promising anticarcingenic agent with less toxicity on healthy tissues at the same time less cancer cell resistance compared to chemotherapeutic drugs.

Keywords

SeNPs; DMBA; Squamous cell carcinoma; Caspase 3

Subject Area

Oral Medicine and Surgical Sciences Issue (Oral Medicine, Oral and Maxillofacial Surgery, Oral Pathology, Oral Biology)

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