Evaluation of the Effect of Different Concentrations of Nanocurcumin on Periodontal Fibroblastic Cell Culture Isolated from Periodontitis Patient

Authors

Thamar A. Bossiela, Dentist at Ministry of Health B.D.S. (2000) Cairo University, Cairo, Egypt.Follow
Naglaa Shawki Elkilani, Professor and Head of Oral Medicine, Periodontology, Oral Diagnosis and Oral Radiology Department Faculty of Dental Medicine for Girls' Al-Azhar University, Cairo, Egypt.
Eman M. Kandeel, Professor and Head of Organic and Applied Chemistry Department, Faculty of Science for Girls' Al-Azhar University, Cairo, Egypt.
Amal A. Ibrahim, Lecturer of Oral Medicine, Periodontology, Oral Diagnosis and Radiology, Faculty of Dental Medicine for Girls Al-Azhar University.

Document Type

Original Study

Abstract

Purpose: The goal of this study was to see how varying concentrations of nanocurcumin (NC) affected the culture of periodontal ligament fibroblastic cells (PDLFCs). Materials and Methods: Nanocurcumin was prepared at concentrations (10 µmol/mL, 3 µmol/mL, 1.5 µmol/mL, 0.7 µmol/mL and , 0.3 µmol/mL ). Periodontal ligament fibroblastic cells were extracted, characterized using Flowcytometry and cultured in Dulbecco's Modified Eagle Medium (DMEM). The healthy PDLFCs served as the positive control group, however the PDLFCs of periodontitis patients were divided into two subgroups. 1st subgroup: PDLFCs treated with NC different concentrations , counted by hemocytometer and Cell proliferation assay was done by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) at 24 ,48 , and 72 hours for calculation of NC half maximal effective concentration ( EC50) at each interval then detection of cell apoptosis when treated with the resultant NC EC50 . 2nd subgroup: untreated cells were used to compare with each result. Results: There was a statistically significant difference in the mean Log10 cell count, cell viability %, and optical density between the 10µmol/mL nanocurcumin and all concentrations of nanocurcumin, and the overall p value for intergroup comparison was highly statistically significant. Conclusion: Nanocurcumin had no in vitro cytotoxicity to PDLFCs at any concentrations used by MTT assay. Nanocurcumin may be a promising medicine for periodontitis treatment and periodontal regeneration.

Keywords

Nanocurcumin, Periodontal ligament Fibroblastic cells, Apoptosis.

How to Cite This Article

Bossiela, Thamar A.; Elkilani, Naglaa Shawki; Kandeel, Eman M.; and Ibrahim, Amal A. (2023) "Evaluation of the Effect of Different Concentrations of Nanocurcumin on Periodontal Fibroblastic Cell Culture Isolated from Periodontitis Patient," Al-Azhar Journal of Dentistry: Vol. 10: Iss. 3, Article 2.
DOI: https://doi.org/10.58675/2974-4164.1548

Subject Area

Oral Medicine and Surgical Sciences Issue (Oral Medicine, Oral and Maxillofacial Surgery, Oral Pathology, Oral Biology)