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Document Type

Original Study

Abstract

The Purpose of this study: was to assess the potential anticarcinogenic of emodin (an aloe vera extract) on tongue squamous cell carcinoma cell line. MATERIALS and METHODS: six groups were included in this study, Group I& II: different concentrations of Emodin and 5FU, respectively, were applied on WI38 normal epithelial cell line. The MTT cytotoxicity test was performed to explore the cytotoxicity of both substances on normal epithelial cells (WI38). Groups III to VI: various concentrations of emodin, 5FU, and combination of both substances with different ratios, respectively, were applied on cultivated SCC25 cell line to choose the concentrations with high cytotoxic effects according to their IC50 values at 48 and 72 hours. Results: IC50 of group I [Emodin/WI38 (106±4.93) & (93.4±3.56)] is higher than group II [5FU/WI38 (49.0±2.29) & (45.8±1.48)] at 48 and 72h , respectively, which means that lower concentration of 5FU is more cytotoxic to normal cells than emodin. While, the highest IC50 mean values were in group III [Emodin/SCC25 (47.0±2.28) &(38.6±3.56)], then group IV [5FU/SCC25 (12.50±0.6)& (7.62±0.29)], followed by group VI [Emodin+ low 5-FU/SCC25 (5.41±0.26)& (6.91±0.26)], with the least value was in group V [Emodin+ high 5-FU/SCC25 (3.29±0.16)& (2.89±0.11)] at 48 and 72h , respectively, which means that addition of emodin to either high or low concentrations of 5FU will decrease the concentration than if 5FU or emodin used alone. Conclusion: adding emodin to either high or low concentrations of 5FU lower the concentration compared to using 5FU or emodin alone.

Keywords

Emodin; Anticarcinogenic; cell line

Subject Area

Oral Medicine and Surgical Sciences Issue (Oral Medicine, Oral and Maxillofacial Surgery, Oral Pathology, Oral Biology)

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