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Document Type

Original Study

Abstract

Objective: This study was to evaluate the activity of Lactate Dehydrogenase enzymein different tooth malpositions in gingival crevicular fluid (GCF). Patients, materialsand methods: A sample of 18 patients with an age range from 13 to 19years, requiringorthodontic treatment. The study groups were designed into two groups. GroupI: well aligned teeth (control group). Group II: malposed teeth. Group II was subsequentlydivided into 3 sub-groups; Rotation, Spacing and Vertical malposition. Sampleswere collected for assessment of lactate dehydrogenase level in GCF at baseline, oneweek, two and four weeks after treatment. Lactate dehydrogenase was measured inGCF using Enzyme Linked Immunosorbent Assay (ELISA) technique. Results: In bothgroups (group I and group II subgroups) the gingival crevicular fluid lactate dehydrogenaseenzyme had a steady increase during orthodontic tooth movement with a statisticallysignificant increase at one week and two weeks compared with the base line(P≤0.05). No statistical significant difference was found between each two subgroupsof group II (P≤ 0.05). Conclusions: The level of lactate dehydrogenase enzyme in gingivalcrevicular fluid could be used as a biomarker for periodontal metabolism relatedto orthodontic tooth movement. The level of lactate dehydrogenase enzyme (LDH) didnot show significant difference among different tooth malpositions during correctionby orthodontic treatment.

Keywords

Lactate dehydrogenase; GCF; ELISA

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