Stem Cells and Their Potential Effect on Irradiated Submandibular Salivary Glands in Mice

Authors

Seham Hallool, Assistant Lecturer of Oral and Dental Biology, Faculty of Dental Medicine for Girls, Al-Azhar University, Cairo, EgyptFollow
Mona Farid, professor of oral and dental biology, Former Dean of the Faculty of Dental Medicine for Girls, Al-Azhar University, Cairo, EgyptFollow
Samah Mehanny, Professor of Oral and Dental Biology, Faculty of Dentistry, Cairo University, Cairo, EgyptFollow
Hanan Abdel Hamid, Assistant Professor of Biochemistry, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority, Cairo, EgyptFollow

Corresponding Author

Hallool, Seham

Document Type

Original Study

Abstract

Purpose: the objective of the present study was to evaluate the effect of BM-MSCs on irradiated submandibular salivary glands in mice and to determine at which stage post-irradiation the cells should be transplanted. Materials and methods: 58 adult male mice were used, 10 for preparation of PKH-26-labeled BM-MSCs and the remaining 48 mice were divided into: control group (GӀ) consisted of 6 mice without radiation and experimental groups consisted of 42 irradiated mice irradiated with a single dose of 15Gy. They were randomly divided into three groups: GӀӀ (+ve control): 18 irradiated not treated mice and two treated groups each consisted of 12 irradiated mice and treated with intraglandular injection of labeled BM-MSCs, 24 hours (GӀӀӀ) and at day 11 (GΙV) post-radiation. Animals were sacrificed at day 6, day 30 and day 90 post-radiation. The submandibular glands were collected, one gland was routinely processed for histological examination using H&E staining and the other was prepared for immunohistochemical study using immunofluorescence staining with anti- α-amylase 1 antibody. Results: BM-MSCs produced marked improvement in salivary gland tissue architecture, histology of parenchymatous and stromal structures and glands function indicated by marked increase in α amylase 1 production compared with +ve control group, with GӀӀӀ showed greater improvement than GΙV. Conclusions: It could be concluded that: intraglandular transplantation of BM-MSCs could successfully regenerate radiation-induced submandibular salivary gland damage and restore the gland function. The early transplantation of BM-MSCs 24 hours post-radiation was markedly more beneficial than the transplantation at day 11 post-radiation.

Keywords

BM-MSCs; Submandibular salivary glands; Radiation; immunofluorescence; anti- α- amylase 1

How to Cite This Article

Hallool, Seham; Farid, Mona; Mehanny, Samah; and Abdel Hamid, Hanan (2020) "Stem Cells and Their Potential Effect on Irradiated Submandibular Salivary Glands in Mice," Al-Azhar Journal of Dentistry: Vol. 7: Iss. 1, Article 5.
DOI: https://doi.org/10.21608/adjg.2019.7619.1099

Subject Area

Oral Medicine and Surgical Sciences Issue (Oral Medicine, Oral and Maxillofacial Surgery, Oral Pathology, Oral Biology)